CEA; Centre National de la Recherche Scientifique (CNRS); Communaute Universite Grenoble Alpes; UDICE-French Research Universities; Universite Grenoble Alpes (UGA); Hiroshima University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
JOURNAL OF GENERAL VIROLOGY
Year
2020
Volume
101
Issue
11
Page
1219-1226
Open Access
Bronze, Green Submitted
Publisher
MICROBIOLOGY SOC
DOI
10.1099/jgv.0.001487
Format
PDF
Abstract
Jumbo phages are bacteriophages that carry more than 200 kbp of DNA. In this study we characterized two jumbo phages (Phi RSL2 and Phi XacN1) and one semi-jumbo phage (Phi RP13) at the structural level by cryo-electron microscopy. Focusing on their capsids, three-dimensional structures of the heads at resolutions ranging from 16 to 9 angstrom were calculated. Based on these structures we determined the geometrical basis on which the icosahedral capsids of these phages are constructed, which includes the accessory and decorative proteins that complement them. A triangulation number novel to Myoviridae (Phi RP13; T=21) was discovered as well as two others, which are more common for jumbo phages (T=27and T=28). Based on one of the structures we also provide evidence that accessory or decorative proteins are not a prerequisite for maintaining the structural integrity of very large capsids.
FRISBI [ANR-10-INBS-05-02]; GRAL within University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS [ANR-17-EURE-0003]; Auvergne-Rhone-Alpes Region; Fondation Recherche Medicale (FRM); fonds FEDER; GIS-Infrastructures en Biologie Sante et Agronomie (IBiSA)
