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PmTBC1D20, a Rab GTPase-activating protein from the black tiger shrimp, Penaeus monodon, is involved in white spot syndrome virus infection


valaiporn - Posted on 14 January 2014

TitlePmTBC1D20, a Rab GTPase-activating protein from the black tiger shrimp, Penaeus monodon, is involved in white spot syndrome virus infection
Publication TypeJournal Article
Year of Publication2014
AuthorsWanchart Yingvilasprasert, Premruethai Supungul, Anchalee Tassanakajon
JournalDevelopmental & Comparative Immunology
Volume42
Issue2
Date Published2/2014
Start Page302
Pagination302 - 310
ISSN0145305X
DOI10.1016/j.dci.2013.09.008
AbstractTBC domain family member 20 (TBC1D20) has recently been reported to mediate Hepatitis C virus replication. Herein, PmTBC1D20 identified from the black tiger shrimp, Penaeus monodon, was characterized and evaluated for its role in white spot syndrome virus (WSSV) infection. The full-length cDNA sequence of PmTBC1D20 contains 2003. bp with a predicted 1443. bp open reading frame encoding a deduced 480 amino acid protein. Its transcript levels were significantly up-regulated at 24 and 48. h by ~2.3- and 2.1-fold, respectively, after systemic infection with WSSV. In addition, depletion of PmTBC1D20 transcript in shrimps by double stranded RNA interference led to a decrease in the level of transcripts of three WSSV genes (VP28, ie1 and wsv477). This suggests the importance of PmTBC1D20 in WSSV infection. This is the first report of TBC1D20 in a crustacean and reveals the possible mechanism used by WSSV to modulate the activity of the host protein, PmTBC1D20, for its benefit in viral trafficking and replication. © 2013 Elsevier Ltd.
KeywordsPenaeus monodon, Rab-GTPase-activating protein, Shrimp, TBC domain, White Spot Syndrome Virus, ศูนย์พันธุวิศวกรรมและเทคโนโลยีชีวภาพแห่งชาติ, สาขาเกษตรศาสตร์และชีววิทยา
Short TitleDevelopmental & Comparative Immunology